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Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker
Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker from Abcam
Be the first to write a review!
Citations:
(>1k)
Supplier Page
Supplier Page from
Abcam for
Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab6326
Antibody Type
Primary
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (PFA perfusion fixed frozen sections), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (PFA perfusion fixed frozen sections)
Clonality
Monoclonal
Quantity
1 MG, 100 UG
Clone
BU1/75 (ICR1)
Host
Rat
Isotype
IgG2a
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Reactivity
Species independent
Target
BrdU
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (1594)
(1)
Astrocytic endothelin-1 overexpression impairs learning and memory ability in ischemic stroke via altered hippocampal neurogenesis and lipid metabolism
Neural Regeneration Research
March 1, 2024
Jie Li, Wen Jiang, Yuefang Cai, Zhenqiu Ning, Yingying Zhou, Chengyi Wang, Sookja Ki Chung, Yan Huang, Jingbo Sun, Minzhen Deng,
et al.
hour, followed by primary antibody
incubation
using rat anti-BrdU (
1:500
,
Abcam
, Boston, MA, USA, Cat#
ab6326
, RRID: AB_305426), mouse anti-SOX2 (
1:200
,
Santa Cruz Biotechnology
, Santa Cruz, CA, USA, Cat#
sc365823
,
(2)
Ethanol changes Nestin-promoter induced neural stem cells to disturb newborn dendritic spine remodeling in the hippocampus of mice
Neural Regeneration Research
February 1, 2024
Guixiang Wang, Wenjia Wang, Ye Zhang, Xiaoying Gou, Qingqing Zhang, Yanmiao Huang, Kuo Zhang, Haotian Zhang, Jingyu Yang, Yuting Li
doublecortin (DCX; rabbit,
1:1000
,
Abcam
, Cat#
Ab207175
, RRID: AB_2894710), BrdU (
rat
,
1:500
,
Abcam
, Cat#
Ab6326
, RRID: AB_305426), neuronal nuclei (NeuN; rabbit,
1:500
,
Merck
, Cat#
MAB377
, RRID: AB_2298772), pS6
(3)
DCAF14 regulates CDT2 to promote SET8-dependent replication fork protection
Life Science Alliance
January 1, 2024
Neysha Tirado-Class, Caitlin Hathaway, Anthony Nelligan, Thuan Nguyen, Huzefa Dungrawala
WB, sc-376377; SantaCruz), ZRANB3 (
1:500
, WB,
A303-033A
;
Bethyl Laboratories
), BrdU-
Rat
(
1:10
, IF,
ab6326
;
Abcam
), BrdU-Ms (
1:100
, IF,
347580
;
BD Biosciences
), Biotin Rb (
1:200
, IF,
A150-109A
;
Bethyl Laboratories
),
(4)
FBXW7-loss sensitizes cells to ATR inhibition through induced mitotic catastrophe
Cancer Research Communications
December 21, 2023
Siobhan O'Brien, Tajinder Ubhi, Lucie Wolf, Krishna Gandhi, Sichun Lin, Naz Chaudary, Neesha C. Dhani, Michael Milosevic, Grant W. Brown, Stephane Angers
immunofluorescence analysis
as described previously (17). CldU was detected using rat anti-BrdU (
Abcam
ab6326
,
1:40
), IdU was detected using mouse anti-BrdU
clone
B44 (
BD Biosciences
#
347580
,
1:10
), and ssDNA was
(5)
Adult stem cell activity in naked mole rats for long-term tissue maintenance
Nat Commun
December 20, 2023
Shamir Montazid, Sheila Bandyopadhyay, Daniel W. Hart, Nan Gao, Brian Johnson, Sri G. Thrumurthy, Dustin J. Penn, Bettina Wernisch, Mukesh Bansal, Philipp M. Altrock,
et al.
primary antibodies used in this study were Chromogranin A (
Abcam
,
ab15160
) at
1:2000
and BrdU (
Abcam
,
ab6326
) at
1:500
. It is noteworthy that in our BrdU
staining
, we did not use HCl-mediated
DNA denaturation
(6)
Targeting EIF4A triggers an interferon response to synergize with chemotherapy and suppress triple-negative breast cancer
Journal of Clinical Investigation
December 15, 2023
Na Zhao, Elena B. Kabotyanski, Alexander B. Saltzman, Anna Malovannaya, Xueying Yuan, Lucas C. Reineke, Nadia Lieu, Yang Gao, Diego A. Pedroza, Sebastian J. Calderon,
et al.
Cell Signaling Technology
[
CST
],
70076S
), anti-
S100A8
(
1:5000
;
R&D Systems
,
MAB3059
), anti-BrdU (
1:1000
;
Abcam
,
ab6326
),
anti–cleaved caspase 3
(
1:1000
;
CST
,
9661
),
anti–phospho-histone H2A.X (Ser139
) (
1:400
;
CST
,
9718
),
(7)
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging
Elife
December 11, 2023
Hirofumi Noguchi, Jessica Chelsea Arela, Thomas T. Ngo, Laura Cocas, Samuel J. Pleasure
chicken
anti-S100B (
1:500
, Synapyic Systems, 287 006, RRID:AB_2713986); rat anti-BrdU (
1:500
,
Abcam
,
ab6326
, RRID:AB_305426) and mouse anti-BrdU (
1:100
,
BD Biosciences
,
347580
, RRID:AB_400326); rabbit anti-GluR2/3
(8)
Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forks
Nucleic Acids Research
December 11, 2023
Yuqin Zhao, Kaiping Hou, Youhang Li, Shuailin Hao, Yu Liu, Yinan Na, Chao Li, Jian Cui, Xingzhi Xu, Xiaohua Wu,
et al.
BRCA2
(
10741S
,
Cell Signaling Technology
),
MRE11
(
ab208020
,
Abcam
),
RAD51
(
ab133534
,
Abcam
), BrdU (
ab6326
,
Abcam
and
347580
,
BD
),
MUS81
(
ab247136
,
Abcam
), BLM (
ab2179
,
Abcam
),
DNA2
(
18727-1-AP
,
Proteintech
),
(9)
Permanent deconstruction of intracellular primary cilia in differentiating granule cell neurons
bioRxiv
December 8, 2023
Carolyn M. Ott, Sandii Constable, Tri M. Nguyen, Kevin White, Wei-Chung Allen Lee, Jennifer Lippincott-Schwartz, Saikat Mukhopadhyay
humid chamber. Primary antibodies: ARL13B (
1:1000
, UC Davis/NeuroMab #75–287), BrdU (
1:500
,
Abcam
#
ab6326
), P27KIP (
1:400
,
BD Biosciences
) #
610241
), and SOX9 (
1:500
,
Millipore
#
ABE571
). For BrdU
staining
we
(10)
Visualizing DNA replication by single-molecule analysis of replicated DNA
STAR Protoc
December 3, 2023
Advaitha Madireddy, Jeannine Gerhardt
1:15
),
Becton Dickinson
,Cat #
347580
Rat
monoclonal anti-bromodeoxyuridine (dilution
1:15
),
Abcam
,Cat #
ab6326
"
Goat anti-rat IgG (H + L) cross-adsorbed
secondary antibody, Alexa Fluor 488 (dilution
1:15
)",
Invitrogen
,Cat
(11)
Nuclear actin polymerization rapidly mediates replication fork remodeling upon stress by limiting PrimPol activity
Nature Communications
November 28, 2023
Maria Dilia Palumbieri, Chiara Merigliano, Daniel González-Acosta, Danina Kuster, Jana Krietsch, Henriette Stoy, Thomas von Känel, Svenja Ulferts, Bettina Welter, Joël Frey,
et al.
tracks were stained for 2.5 h at RT using two different anti-BrdU antibodies recognizing CldU (
Abcam
,
ab6326
,
1:500
) and IdU (
Becton Dickinson
,
347580
,
1:100
), respectively. After washing five times with PBST
(12)
Autophagy drives the conversion of developmental neural stem cells to the adult quiescent state
Nature Communications
November 24, 2023
Isabel Calatayud-Baselga, Lucía Casares-Crespo, Carmina Franch-Ibáñez, José Guijarro-Nuez, Pascual Sanz, Helena Mira
Novus
,
NBP3-15794
), GFP (
1:200
,
Aves
-Lab,
GFP-1010
),
GFAP
(
1:300
,
Sigma
,
G3893
), BrdU (
1:300
,
Abcam
,
ab6326
), MCM2 (
1:150
, BD Biosciences, 610700). Treatment with 2 N HCl was required before
BrdU
detection. Secondary
(13)
Characterization of BRCA2 R3052Q variant in mice supports its functional impact as a low-risk variant
Cell Death & Disease
November 18, 2023
Arun Prakash Mishra, Suzanne Hartford, Rajani Kant Chittela, Sounak Sahu, Suhas S. Kharat, Lucia Alvaro-Aranda, Aida Contreras-Perez, Teresa Sullivan, Betty K. Martin, Mary Albaugh,
et al.
min with 5% BSA in PBST. The fibers were then incubated with primary rat anti-BrdU antibody (
Abcam
ab6326
,
1:500
) and mouse anti-BrdU antibody (
Becton Dickinson
347580
,
1:500
) for 2.5 h in the blocking solution.
(14)
The splicing factor DHX38 enables retinal development through safeguarding genome integrity
iScience
November 17, 2023
Kui Sun, Yunqiao Han, Jingzhen Li, Shanshan Yu, Yuwen Huang, Yangjun Zhang, Jamas Reilly, Jiayi Tu, Pan Gao, Danna Jia,
et al.
antibodies: mouse anti-BrdU antibody (
Becton Dickinson
, Cat#
347580
) and rat anti-BrdU antibody (
Abcam
Cat#
Ab6326
), and then diluted in blocking solution and applied at 4°C overnight. Then, slides were washed twice
(15)
Inactivation of the tumor suppressor gene Apc synergizes with H. pylori to induce DNA damage in murine gastric stem and progenitor cells
Science Advances
November 17, 2023
Jiazhuo He, Zuzana Nascakova, Peter Leary, Giovanni Papa, Tomas Valenta, Konrad Basler, Anne Müller
the dark, at room temperature, with anti–5-bromo-2′-deoxyuridine antibodies recognizing CldU (
rat
,
ab6326
,
Abcam
;
1:500
) and IdU (
mouse
,
347580
B44,
BD Biosciences
;
1:100
), followed by 1 hour of
incubation
(16)
Mouse primary follicles experience slow growth rates after activation and progressive increases that influence the duration of the primary follicle phase†
Biology of Reproduction
November 15, 2023
Sharon Richard, Nicholas J Anderson, Yiran Zhou, Michael W Pankhurst
PBS, with 0.2%
Tween 20
(
Merck
, cat# P1379) for 20 min. Primary antibody (rat anti-BrdU,
Abcam
, cat#:
ab6326
, RRID: AB_305426) was applied at 2 μg/mL PBS with 0.2% w/v
Tween 20
(Sigma-Aldrich, Cat# P1379) and
(17)
Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS
Journal of Neuroinflammation
November 6, 2023
Linda T. Nguyen, Andrea Aprico, Eze Nwoke, Alexander D. Walsh, Farrah Blades, Raphael Avneri, Elodie Martin, Bernard Zalc, Trevor J. Kilpatrick, Michele D. Binder
incorporated BrdU identified using immunostaining as previously described using an anti-BrdU antibody (
1:250
,
ab6326
,
Abcam
, Cambridge, UK) [20]. Coverslips were mounted onto glass microscope slides using Dako fluorescent
(18)
Maternal High Fat Diet in Lactation Impacts Hypothalamic Neurogenesis and Neurotrophic Development, Leading to Later Life Susceptibility to Obesity in Male but Not Female Mice
Advanced Science
October 22, 2023
Yanchao Xu, Dengbao Yang, Lu Wang, Elżbieta Król, Mohsen Mazidi, Li Li, Yi Huang, Chaoqun Niu, Xue Liu, Sin Man Lam,
et al.
phosphate‐buffered saline (PBS; P4417, Sigma), and then incubated in primary rat anti‐BrdU antibody (
1:200
,
ab6326
,
Abcam
) diluted in 1% BSA with PBS plus 0.1% Triton X‐100 (blocking buffer) overnight (12 to 16 hours)
(19)
Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration
iScience
October 20, 2023
Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou,
et al.
Na2B4O7 in water, PH8.5) for 15 min, prior to primary antibody
incubation
. An anti-BrdU antibody (
1:250
,
ab6326
,
Abcam
, UK) was incubated in blocking solution overnight at 4°C, and appropriate secondary antibody
(20)
miR-28-based combination therapy impairs aggressive B cell lymphoma growth by rewiring DNA replication
Cell Death & Disease
October 18, 2023
Teresa Fuertes, Emigdio Álvarez-Corrales, Carmen Gómez-Escolar, Patricia Ubieto-Capella, Álvaro Serrano-Navarro, Antonio de Molina, Juan Méndez, Almudena R. Ramiro, Virginia G. de Yébenes
labeled tracks, fibers were incubated with primary antibodies anti-CldU (
rat
monoclonal anti-BrdU,
Abcam
,
AB6326
), anti-IdU (mouse monoclonal anti-BrdU,
BD Biosciences
,
347580
) and anti-ssDNA (
mouse
monoclonal IgG2a,
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Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker from Abcam
Supplier Page
View Supplier Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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